Scientific Studies 2019

Introductory evaluation of somatic hybrids Solanum pinnatisectum + Solanum tuberosum cv. Keřkovské rohlíčky in terms of morphology and resistance to Phytophthora infestans (A)
Marie Greplová, Jiří Ptáček, Anna Doležalová, Romana Sedmíková, Jaroslava Domkářová
Application of next generation genotyping methods for potato practice
Jiří Ptáček, Jan Šafář, Viktor Kopačka, Renata Švecová, Jaroslava Domkářová, Miroslava Čeplová, Alena Krpálková
Molecular probing of Potato Virus S genome (A)
Jiří Ptáček, Jaroslav Matoušek, Renata Švecová, Jaroslava Domkářová
Origin and development of new genotypes using MAS (marker assisted selection)
Renata Švecová, Jiří Ptáček, Jaroslava Domkářová
Occurrence of silver scurf (Helminthosporium solani) on potato tubers in soil and during storage in the years 2017 and 2018
Ervín Hausvater, Petr Doležal, Petra Baštová, Vladimíra Sedláková, David Hájek

Introductory evaluation of somatic hybrids Solanum pinnatisectum + Solanum tuberosum cv. Keřkovské rohlíčky in terms of morphology and resistance to Phytophthora infestans (A)

GREPLOVÁ, M. – PTÁČEK, J. – DOLEŽALOVÁ, A. – SEDMÍKOVÁ, R. – DOMKÁŘOVÁ, J.
Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod, 2019, 25: 9–22

Interspecific somatic hybrids of wild species S. pinnatisectum PI320342 (2n = 2x = 24, 1EBN) and regional cultivar S. tuberosum Keřkovské rohlíčky (2n = 4s = 48, 4EBN) were evaluated in terms of ability for acclimatization, plant morphology, tuberization, and resistance to Phytophthora infestans in an in vitro test. The percentage of affected leaf area was used for evaluation (AUDPC). All somatic hybrids employed in this study were hexaploids (2n = 6x = 72), all were acclimatized and tuber bearing. In vitro test of resistance to PI showed strong resistance of wild parent and susceptibility of cultivated parent. Somatic hybrids varied between parental genotypes: one of them showed high level of resistance while two displayed susceptibility, the remaining had an intermediate character. Somatic hybrids seem to be promising breeding material.

electrofusion; resistance; P. infestans; potato; breeding; EBN; AUDPC

Corresponding author:
Ing. Marie GREPLOVÁ, Ph.D., Potato Research Institute Havlíčkův Brod,
Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
phone: +420 569 466 239, e-mail: greplova@vubhb.cz

Application of next generation genotyping methods for potato practice

PTÁČEK, J. – ŠAFÁŘ, J. – KOPAČKA, V. – ŠVECOVÁ, R. – DOMKÁŘOVÁ, J. – ČEPLOVÁ, M. – KRPÁLKOVÁ, A.
Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod, 2019, 25: 23-28

Within NCCTN01000062 „Biotechnology Center for Plant Genotyping“ we coordinate in PRI Havlíčkův Brod Partial project TN01000062/04 – Potatoes in cooperation with IEB AS CR, v.v.i. and VESA Velhartice
The project execution started in 2019 and we present here the results achieved so far.

DNA; DArT; genotyping; potato

Corresponding author:
RNDr. Jiří PTÁČEK, CSc., Potato Research Institute Havlíčkův Brod
Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
phone.: +420 569 466 244, e-mail: ptacek@vubhb.cz

Molecular probing of Potato Virus S genome (A)

PTÁČEK, J. – MATOUŠEK, J. – ŠVECOVÁ, R. – DOMKÁŘOVÁ, J.
Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod, 2019, 25: 29-38

Complete genomes of three isolates of Potato virus S (PVS) were cloned and sequenced. The complete sequence of PVS genome enabled to develop an immunocapture RT-PCR probing of the PVS genome. Using this system, the sequence variability of 11 genome zones was examined for 34 PVS isolates including 15 PVS-CS variants that caused a systemic infection in Chenopodium quinoa. A broad variability between PVS isolates and diverse sequence variants was found. cDNA fragments covering the coat protein (CP) leader and CP-coding region (approx. 420 bp) were pooled for PVS-O and Chenopodium-systemic PVS isolates (PVS-CS) and corresponding cDNA libraries were screened for sequence variants. Genome probing suggested that the PVS-CS isolates analyzed were close to the ordinary European isolates of ordinary strain of PVS (PVS-O) but distant to the original Andean strain of PVS (PVS-A).

genome sequence; Potato virus S; RT-PCR; Solanum tuberosum L.; virus genome analysis

Origin and development of new genotypes using MAS (marker assisted selection)

ŠVECOVÁ, R. – PTÁČEK, J. – DOMKÁŘOVÁ, J.
Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod, 2019, 25: 39-50

We managed to obtain characterized hybrid materials using wild species of the Solanum genus, which have a large amount of genetic diversity for economically important traits. These materials will be used next year for testing for selected properties, for morphological evaluation and for further backcrossing. Prospective materials will be transferred to the gene bank of the Potato Research Institute in Havlíčkův Brod.

wild species; RAPD

Corresponding author:
Ing. Renata ŠVECOVÁ, Potato Research Institute Havlíčkův Brod
Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
phone: +420 569 466 225, e-mail: svecova@vubhb.cz

Occurrence of silver scurf (Helminthosporium solani) on potato tubers, in soil and during storage in the years 2017 and 2018

HAUSVATER, E. – DOLEŽAL, P. – BAŠTOVÁ, P. – SEDLÁKOVÁ, V. – HÁJEK, D.
Vědecké práce – Výzkumný ústav bramborářský Havlíčkův Brod, 2019, 25: 53-66

Silver scurf infection of potato tubers was studied for several varieties in exact field trials in connection with harvest dates between the years 2016 and 2018 and further in two potato stores during storage season 2018/2019. The number of infected tubers and silver scurf severity significantly increased with later harvest date. An increase in silver scurf occurrence was also determined during storage period. This trend was clear in all studied years and all varieties. Considering the fact that silvers scurf is an important disease negatively impacting internal quality of ware potatoes and that direct fungicide control is currently not real, the selected harvest date could be an important preventive measure against this pathogen.

potato silver scurf; tuber infection; harvest date; storage

Corresponding author:
Ing. Ervín HAUSVATER, CSc., Potato Research Institute Havlíčkův Brod
Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic
phone: +420 569 466 237, e-mail: hausvater@vubhb.cz